In order to survive within a host organism, pathogenic bacteria have evolved flexible metabolic pathways and defense mechanisms. Dr. Frawley uses the model pathogen Salmonella Typhimurium to study how bacteria respond to host-generated chemical defenses such as nitric oxide and hydrogen peroxide. Many key enzymes and pathways are directly inhibited by peroxide and nitric oxide so, in order to survive, bacteria must find ways to adapt to, and repair damage that occurs. Dr. Frawley is particularly interested in how nitric oxide affects metal-containing proteins and how metal transport impacts bacterial stress resistance. Current work focuses on the roles that bacterial copper and manganese transporters play during infection and how the function of these proteins is regulated.
Frawley ER, Karlinsey JE, Singhal A, Libby SJ, Paschalis-Thomas D, Ischiropoulos H, Fang FC. 2018. Nitric oxide disrupts zinc homeostasis in Salmonella enterica serovar Typhimurium. mBio. 9(4) doi:10.1128/mBio.01040-18 Fang FC, Frawley ER, Tapscott T, Vazquez-Torres A. 2016. Discrimination and Integration of Stress Signals by Pathogenic Bacteria. Cell Host and Microbe. 20(2): 144-53.
Fang FC, Frawley ER, Tapscott T, Vazquez-Torres A. 2016. Bacterial Stress Responses during Host Infection. Cell Host and Microbe. 20(2): 133-43.
Frawley ER and Fang FC. 2014. The ins and outs of bacterial iron metabolism. Molecular Microbiology. 93(4): 609-616.
Frawley ER, Crouch ML, Bingham-Ramos LK, Robbins HF, Wang W, Wright GD, Fang FC. 2013. Iron and Citrate Export by a Major Facilitator Superfamily Pump Regulates Metabolism and Stress Resistance in Salmonella Typhimurium. PNAS. 110(29): 12054-12059.
Karlinsey JE, Bang IS, Becker LA, Frawley ER, Porwollik S, Robbins HF, Thomas VC, Urbano R, McClelland M, Fang FC. 2012. The NsrR Regulon in Nitrosative Stress Resistance of Salmonella enterica serovar Typhimurium. Molecular Microbiology. 85(6): 1179-1193.
Frawley, ER and Kranz, RG. 2009. CcsBA is a cytochrome c synthetase that also functions in heme transport. PNAS. 106(25): 10201-10206.
BA: Biology, Washington University 2003